Cameron P U, Hunter S D, Jolley D, Sonza S, Mijch A, Crowe S M
Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia.
Cytometry. 1998 Sep 1;33(1):83-8.
The clinical utility of a flow cytometric assay (FCA) for intracellular HIV p24 antigen was evaluated in a group of HIV-1-infected subjects. A previously described method, p24-FCA (1), was modified to minimize nonspecific staining and to include irrelevant isotype-matched control antibodies. Blood mononuclear cells from 32 HIV-1 seropositive subjects and 14 HIV-1 seronegative controls were examined. In HIV-1 seropositive individuals, the proportion of CD4+ lymphocytes that bound the p24 monoclonal and the isotype control antibodies were not different. The frequency of cells binding p24 antibodies increased with declining CD4 counts and was highest in patients with low CD4+ lymphocyte counts. Although results of p24-FCA do reflect disease progression, the high nonspecific binding of monoclonal antibodies in infected subjects obscures specific p24 binding and precludes its use as an accurate measure of infection within single cells.
在一组HIV-1感染受试者中评估了用于检测细胞内HIV p24抗原的流式细胞术检测法（FCA）的临床实用性。对先前描述的p24-FCA方法（1）进行了改进，以尽量减少非特异性染色，并纳入无关的同型匹配对照抗体。检测了32名HIV-1血清阳性受试者和14名HIV-1血清阴性对照者的血液单核细胞。在HIV-1血清阳性个体中，结合p24单克隆抗体和同型对照抗体的CD4 +淋巴细胞比例没有差异。结合p24抗体的细胞频率随CD4计数下降而增加，在CD4 +淋巴细胞计数低的患者中最高。尽管p24-FCA的结果确实反映了疾病进展，但感染受试者中存在的单克隆抗体高非特异性结合掩盖了特异性p24结合，使其无法用作单细胞内感染的准确测量方法。
