Pirker M E, Montedonico S, Rolle U, Austvoll H, Puri P
Children's Research Centre, Our Lady's Hospital for Sick Children and University College Dublin, Dublin, Ireland.
Pediatr Surg Int. 2005 Mar;21(3):161-8. doi: 10.1007/s00383-004-1313-5. Epub 2004 Nov 27.
Nitric oxide (NO) is involved in normal bladder physiology by regulating local arteriolar tone and smooth muscle relaxation and modulating the production of extracellular matrix proteins in vitro. Little information is available regarding the nitrergic innervation of the bladder during development. In this study we investigated the changes in density and morphology of the intramural nitrergic neurons of the porcine urinary bladder during development using whole-mount preparation. Bladder specimens were obtained from porcine foetuses of gestational age 60 days (n=5) and 90 days (n=5) and from newborn piglets (n=5) after perfusion fixation. Bladders were divided into base, body, and dome. Whole-mount preparation using NADPH-diaphorase (NADPH-d) histochemistry was used to visualize nitrergic innervation of the urinary bladders and to measure density of NADPH-positive ganglia (including single neurons), number of NADPH-d positive neurons per ganglion, and size of individual neurons. One-way ANOVA and chi-square tests were used for statistical analysis with a p-value <0.05 considered statistically significant. NADPH-d positive ganglia were numerous in the muscular layer of all three age groups. At E60, ganglion density was significantly higher in the body (mean 880/cm(2)) than in the dome (397/cm(2)) or the base (676/cm(2)). The ganglion density significantly decreased with age. The number of NADPH-d positive neurons per ganglion increased significantly between E90 and birth (p<0.01). A marked increase in the size of individual neurons over time was also seen (p<0.001), predominantly due to an increase in cytoplasm. Our data on whole-mount preparations demonstrate that significant maturation in nitrergic neuronal density and morphology occurs in the porcine urinary bladder, at least until birth.
一氧化氮（NO）通过调节局部小动脉张力和平滑肌松弛以及在体外调节细胞外基质蛋白的产生，参与正常膀胱生理功能。关于发育过程中膀胱的氮能神经支配的信息很少。在本研究中，我们使用整装标本研究了猪膀胱壁内氮能神经元在发育过程中的密度和形态变化。在灌注固定后，从妊娠60天（n = 5）和90天（n = 5）的猪胎儿以及新生仔猪（n = 5）获取膀胱标本。膀胱分为底部、体部和顶部。使用还原型辅酶II-黄递酶（NADPH-d）组织化学的整装标本用于观察膀胱的氮能神经支配，并测量NADPH阳性神经节（包括单个神经元）的密度、每个神经节中NADPH-d阳性神经元的数量以及单个神经元的大小。使用单因素方差分析和卡方检验进行统计分析，p值<0.05被认为具有统计学意义。在所有三个年龄组的肌肉层中，NADPH-d阳性神经节都很多。在胚胎60天（E60）时，体部的神经节密度（平均880个/cm²）显著高于顶部（397个/cm²）或底部（676个/cm²）。神经节密度随年龄显著降低。每个神经节中NADPH-d阳性神经元的数量在E90和出生之间显著增加（p<0.01）。随着时间的推移，单个神经元的大小也显著增加（p<0.001），主要是由于细胞质增加。我们关于整装标本的数据表明，至少在出生前，猪膀胱的氮能神经元密度和形态发生了显著成熟。
