Lee HeaYeon, Park JongWan, Jung HoSup, Kim JongMin, Kawai Tomoji
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
J Nanosci Nanotechnol. 2004 Sep;4(7):882-5. doi: 10.1166/jnn.2004.103.
The assay of DNA biosensor-based nucleic acid recognition using microfabrication technology provides for high sensitivity, good surface coverage and reproducibility. We have achieved efficient immobilization and hybridization of nonlabeled DNA using cyclic voltammetry (CV), square wave voltammetry (SWV) and scanning near-field optical microscopy (SNOM) techniques. The increased electrochemical response observed following the immobilization of biotinlyated ssDNA probe suggests that nucleic acid is a somewhat better medium for electronic transfer. We demonstrated the high coverage of immobilized FITC-labeled biotinylated DNA probe on a streptavidin-modified surface using SNOM imaging. SNOM imaging of FITC-labeled complementary DNA also exhibited fluorescent light spots of hybridization distributed throughout. No fluorescent light was observed with the hybridization of non-complementary DNA.
利用微加工技术对基于DNA生物传感器的核酸识别进行分析，可实现高灵敏度、良好的表面覆盖率和重现性。我们已通过循环伏安法（CV）、方波伏安法（SWV）和扫描近场光学显微镜（SNOM）技术实现了未标记DNA的高效固定和杂交。固定生物素化单链DNA探针后观察到的电化学响应增强表明，核酸是一种稍好的电子转移介质。我们使用SNOM成像证明了FITC标记的生物素化DNA探针在链霉亲和素修饰表面上的高覆盖率。FITC标记的互补DNA的SNOM成像也显示杂交荧光亮点遍布各处。非互补DNA杂交未观察到荧光。
