抗生物素蛋白-生物素技术与吸附转移溶出方波伏安法在转基因抗生物素蛋白玉米中DNA杂交及抗生物素蛋白检测中的应用

Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize.

作者信息

Masarik Michal, Kizek Rene, Kramer Karl J, Billova Sabina, Brazdova Marie, Vacek Jan, Bailey Michele, Jelen Frantisek, Howard John A

机构信息

Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-612 65 Brno, Czech Republic.

出版信息

Anal Chem. 2003 Jun 1;75(11):2663-9. doi: 10.1021/ac020788z.

DOI:10.1021/ac020788z

PMID:12948134

Abstract

The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.

摘要

通过碳糊电极（CPE）上的吸附转移溶出方波伏安法（AdTS SWV）对溶液中的链霉亲和素和抗生物素蛋白进行了电化学检测。AdTS SWV用于定量生物素化寡核苷酸、DNA杂交以及转基因抗生物素蛋白玉米提取物中的抗生物素蛋白。变性链霉亲和素和天然链霉亲和素的检测限分别为6 pM和120 nM。结果表明，链霉亲和素/抗生物素蛋白AdTS SWV是一种灵敏且特异的方法，可用于定量食品和组织提取物等生物样品中的DNA和蛋白质，包括转基因作物（抗生物素蛋白玉米）及邻近田地中的其他植物。