胶束电动毛细管电泳法测定大黄及其茶制剂中的活性蒽醌类成分

[Determination of active anthraquinones in Rheum and its tea preparations by micellar electrokinetic capillary electrophoresis].

作者信息

Zheng Wen-jie, Chen Xing-guo, Jia Wei

机构信息

School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2004 Sep;29(9):870-3.

PMID:15575207

Abstract

OBJECTIVE

To establish an instant determination method of emodin, aloe-emodin and rhein, from Rheum, and one of their preparations, Qinghai Wild Dahuang Tea, by micellar electrokinetic capillary electrophoresis for the first time.

METHOD

Separation was carried out in an uncoated fused silica capillary (75 microm x 50.0 cm). Meanwhile, a running voltage 20 kV, 15.0 mmol x L(-1) borax buffer with 30.0 mmol x L(-1) SDS and 10% ethanol (pH 9.60) and a UV detector at 254 nm were adopted.

RESULT

The linear calibration rang was 4-120 mg x L(-1) (r = 0.992 1) for emodin, 10-200 mg x L(-1) (r = 0.997 0) for aloe-emodin, and 2-100 mg x L(-1) (r = 0.997 1) for rhein, respectively. Under the optimum conditions, the relative standard deviation (RSD) values (n = 6) for the migration time and the peak area of each peak were 0.59%-0.80%, 1.30%-3.22%, respectively. The contents of the analytes were easily determined with recoveries ranging from 97.6%-102.3%.

CONCLUSION

The method is proved to be simple, rapid and accurate, and can be used for the quality control of medicinal herb, Rheum, and its tea preparation.

摘要

目的

首次建立采用胶束电动毛细管电泳法快速测定大黄及其制剂青海野生大黄茶中大黄素、芦荟大黄素和大黄酸的方法。

方法

采用未涂层熔融石英毛细管（75μm×50.0cm）进行分离。同时，采用运行电压20kV，15.0mmol·L⁻¹硼砂缓冲液（含30.0mmol·L⁻¹十二烷基硫酸钠和10%乙醇，pH9.60），以及254nm紫外检测器。

结果

大黄素的线性校准范围为4 - 120mg·L⁻¹（r = 0.9921），芦荟大黄素为10 - 200mg·L⁻¹（r = 0.9970），大黄酸为2 - 100mg·L⁻¹（r = 0.9971）。在最佳条件下，各峰迁移时间和峰面积的相对标准偏差（RSD）值（n = 6）分别为0.59% - 0.80%、1.30% - 3.22%。分析物含量易于测定，回收率在97.6% - 102.3%之间。