Campus Guglielmo, Lallai Maria Rosaria, Carboni Roberto, Spano Giovanni, Luglié Pierfranca, García-Godoy Franklin
Dental Institute, University of Sassari, Viale San Pietro 43/C, 1-07100 Sassari, Sardinia, Italy.
Am J Dent. 2004 Oct;17(5):351-3.
To evaluate fluoride uptake in enamel after use of products containing different fluoride components of distinct oral hygiene regimen in vivo.
84 healthy subjects (24 females and 60 males aged 19-26 years, mean 22.2) recruited from the students attending the courses of the Medical Faculty of the University of Sassari, Italy, were randomly assigned to one of four treatments groups: A: treated with a dentifrice containing sodium monofluorophosphate (1250 ppm F-); B: treated with dentifrice with 1250 ppm F- from AmF; C: treated with an AmF toothpaste (1250 ppm F-) and a mouth rinse with 250 ppm F- from AmF; or D: treated with a NaMFP toothpaste (1250 ppm F-) plus 1250 ppm NaMFP varnish. Acid-etched enamel microbiopsies on the vestibular surface of the first maxillary premolar were collected at baseline (to), after 20 days of test product use (t1) and after 24 hours without treatment (t2). Fluoride concentration was measured using an ion-specific electrode. All measurements were made in triplicate and analyzed statistically using two-way factorial ANOVA for independent samples standard weighted-means analysis.
The fluoride uptake in the enamel shows a considerable variation between the groups. Significant differences were found both within all products in terms of fluoride concentration in enamel for (P < 0.0001 for two-way ANOVA, and later Tukey HSD test). After 20 days of treatment, the group using AmF toothpaste plus AmF mouthrinse had a higher fluoride uptake than the groups using either of the other products (P < 0.05). In conclusion, all products lead to an appreciable amount of fluoride uptake in enamel; AmF products led to higher concentrations. The concentration of fluoride accumulated during treatment phase remained high after 24 hours following the cessation of use.
在体内评估使用含有不同氟成分且口腔卫生方案不同的产品后牙釉质中的氟摄取情况。
从意大利萨萨里大学医学院课程的学生中招募84名健康受试者（24名女性和60名男性，年龄19 - 26岁，平均22.2岁），随机分配到四个治疗组之一：A组：用含单氟磷酸钠（1250 ppm F-）的牙膏治疗；B组：用含1250 ppm F-的氟化亚锡牙膏治疗；C组：用含1250 ppm F-的氟化亚锡牙膏和含250 ppm F-的氟化亚锡漱口水治疗；或D组：用含1250 ppm F-的单氟磷酸钠牙膏加1250 ppm单氟磷酸钠 varnish治疗。在基线（t0）、使用测试产品20天后（t1）和停止治疗24小时后（t2），采集上颌第一前磨牙前庭表面的酸蚀牙釉质活检样本。使用离子特异性电极测量氟浓度。所有测量均重复三次，并使用双向析因方差分析进行独立样本标准加权均值分析的统计学分析。
牙釉质中的氟摄取在各组之间显示出相当大的差异。在所有产品中，就牙釉质中的氟浓度而言均发现了显著差异（双向方差分析P < 0.0001，随后进行Tukey HSD检验）。治疗20天后，使用氟化亚锡牙膏加氟化亚锡漱口水的组比使用其他任何一种产品的组具有更高的氟摄取（P < 0.05）。总之，所有产品都会导致牙釉质中摄取相当量的氟；氟化亚锡产品导致更高的浓度。在停止使用24小时后，治疗阶段积累的氟浓度仍然很高。
