Chun Ki Woo, Yoo Hyuk Sang, Yoon Jun Jin, Park Tae Gwan
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
Biotechnol Prog. 2004 Nov-Dec;20(6):1797-801. doi: 10.1021/bp0496981.
Poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres were prepared by an oil/water emulsion solvent evaporation method to use as an injectable microcarrier for cell delivery. Three different kinds of PLGA microspheres having hydrophobic, negatively charged, and positively charged surfaces were prepared. Hydrophobic and negatively charged PLGA microspheres were prepared by using terminally capped and uncapped PLGA polymer, respectively. Positively charged PLGA microspheres were prepared by blending PLGA with PLGA-g-poly(L-lysine) graft copolymer as a surface modifying agent. Bovine chondrocytes were cultured on the three PLGA microspheres under serum conditions to comparatively evaluate cell attachment, cell proliferation, and cell function with respect to surface properties. Positively charged PLGA microspheres showed the highest cell attachment, growth, and function compared to hydrophobic and negatively charged microspheres. Surface-modified PLGA microspheres can potentially be used as an injectable delivery system for cells into a tissue defect site.
采用油/水乳液溶剂蒸发法制备了聚（D，L-乳酸-共-乙醇酸）（PLGA）微球，用作细胞递送的可注射微载体。制备了具有疏水、带负电荷和带正电荷表面的三种不同类型的PLGA微球。疏水和带负电荷的PLGA微球分别通过使用封端和未封端的PLGA聚合物制备。带正电荷的PLGA微球通过将PLGA与作为表面改性剂的PLGA-g-聚（L-赖氨酸）接枝共聚物共混制备。在血清条件下，将牛软骨细胞培养在三种PLGA微球上，以比较评估细胞附着、细胞增殖和细胞功能与表面性质的关系。与疏水和带负电荷的微球相比，带正电荷的PLGA微球表现出最高的细胞附着、生长和功能。表面改性的PLGA微球有可能用作将细胞注射到组织缺损部位的递送系统。
