Choi Ha-Jong, Kim Chang Sup, Kim Pil, Jung Heun-Chae, Oh Deok-Kun
Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
Biotechnol Prog. 2004 Nov-Dec;20(6):1876-9. doi: 10.1021/bp049770v.
Lactosucrose, a functional trisaccharide, was produced from lactose as an acceptor and sucrose as a fructosyl donor by whole cells harboring transfructosylation activity of levansucrase. Levansucrase-induced cells of Paenibacillus polymyxa were obtained in the medium containing sucrose, and the transfructosylation activity in the whole cell was optimized for lactosucrose production. The optimal cell concentration, substrates ratio, temperature, and pH were 2.0% (w/v), 22.5% (w/v) lactose and 22.5% (w/v) sucrose, 55 degrees C, and 6.0, respectively. Under these conditions, the whole cells produced approximately 17.0% (w/v) lactosucrose in 6 h of reaction time with a productivity of 2.8% (w/v)/h.
乳蔗糖是一种功能性三糖，由具有果聚糖蔗糖酶转果糖基化活性的全细胞以乳糖为受体、蔗糖为果糖基供体生产得到。在含有蔗糖的培养基中获得了由果聚糖蔗糖酶诱导的多粘芽孢杆菌细胞，并对全细胞中的转果糖基化活性进行了优化以生产乳蔗糖。最佳细胞浓度、底物比例、温度和pH分别为2.0%（w/v）、22.5%（w/v）乳糖和22.5%（w/v）蔗糖、55℃和6.0。在这些条件下，全细胞在6小时的反应时间内产生了约17.0%（w/v）的乳蔗糖，生产率为2.8%（w/v）/小时。
