Miguel Graça, Fontes Catarina, Antunes Dulce, Neves Alcinda, Martins Denise
J Biomed Biotechnol. 2004;2004(5):338-342. doi: 10.1155/S1110724304403076.
The concentration of anthocyanins in fruits of "Assaria" pomegranate, a sweet Portuguese cultivar typically grown in Algarve (south Portugal), was monitored during storage under different conditions. The fruits were exposed to cold storage ( $5^{\circ}$ C) after the following treatments: spraying with wax; spraying with $1.5$ % CaCl(2); spraying with wax and $1.5$ % CaCl(2); covering boxes with 25 $\mu$ c thickness low-density polyethylene film. Untreated fruits were used as a control. The anthocyanin levels were quantified by either comparison with an external standard of cyanidin 3-rutinoside (based on the peak area) or individual calculation from the peak areas based on standard curves of each anthocyanin type. The storage time as well as the fruit treatment prior to storage influenced total anthocyanin content. The highest levels were observed at the end of the first month of storage, except for the fruits treated with CaCl(2), where the maximal values were achieved at the end of the second month. The anthocyanin quantification method influenced the final result. When total anthocyanin was calculated as a sum of individual pigments quantified based on standard curves of each anthocyanin type, lower values were obtained.
“阿萨里亚”石榴是一种典型生长于葡萄牙阿尔加维地区（葡萄牙南部）的甜味葡萄牙品种，在不同条件下储存期间，对其果实中的花青素浓度进行了监测。果实经过以下处理后进行冷藏（5℃）：喷洒蜡；喷洒1.5%的氯化钙；喷洒蜡和1.5%的氯化钙；用厚度为25微米的低密度聚乙烯薄膜覆盖箱子。未处理的果实用作对照。花青素含量通过与矢车菊素3 - 芸香糖苷外标比较（基于峰面积）或根据每种花青素类型的标准曲线从峰面积单独计算来定量。储存时间以及储存前的果实处理会影响总花青素含量。除了用氯化钙处理的果实，在储存第一个月末观察到最高含量，用氯化钙处理的果实在第二个月末达到最大值。花青素定量方法会影响最终结果。当根据每种花青素类型的标准曲线将总花青素计算为单个色素的总和时，得到的值较低。
