da Costa C T, Horton D, Margolis S A
Evora University, Department of Chemistry, Portugal.
J Chromatogr A. 2000 Jun 9;881(1-2):403-10. doi: 10.1016/s0021-9673(00)00328-9.
This article reviews recent developments in the methodology for the measurement of anthocyanins that offer several advantages over classical methods of analysis. The use of UV-diode array and mass spectrometric (MS) detectors, with improved methods of liquid chromatography analysis has facilitated identification of these analytes. The use of capillary electrophoresis (CE) analysis of the anthocyanins under acid conditions has significantly increased peak resolution and improved the detection limits by several orders of magnitude. CE offers the advantage of economies of very small sample size, very small solvent consumption, and short analysis times along with the future possibility of being combined with MS detection.
本文综述了花青素测量方法的最新进展，这些方法相较于传统分析方法具有诸多优势。紫外二极管阵列和质谱（MS）检测器的使用，以及液相色谱分析方法的改进，有助于这些分析物的鉴定。在酸性条件下对花青素进行毛细管电泳（CE）分析，显著提高了峰分辨率，并将检测限提高了几个数量级。CE具有样品用量极少、溶剂消耗极少、分析时间短的优点，并且未来有可能与MS检测相结合。
