dos Santos Pierre Alexandre, Amarante Maria Fernanda Castro, Pereira Ana Maria Soares, Bertoni Bianca, França Suzelei Castro, Pessoa Cláudia, de Moraes Manoel Odorico, Costa-Lotufo Letícia Veras, Pereira Marcio Roberto Pinho, Lopes Norberto Peporine
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão, Preto-SP, Brazil.
Chem Pharm Bull (Tokyo). 2004 Dec;52(12):1433-5. doi: 10.1248/cpb.52.1433.
This work reports for the first time the production a furanoheliangolide (goyazensolide) by plant cell culture. Monitoring of the goyazensolide metabolism revealed that the maximum production occurred during the lag phase of the Lychnophora ericoides callus culture. The antiproliferative activity of obtained goyazensolide was evaluated against seven cancer cell lines using MTT assay. The results revealed a potent cytotoxic activity for the furaheliangolide with IC50 values in the range of 0.06 microg/ml for CEM leukemia cells to 0.75 microg/ml for B16 melanome cells.
这项工作首次报道了通过植物细胞培养生产呋喃合亮内酯（戈亚森内酯）。对戈亚森内酯代谢的监测表明，其最大产量出现在绒毛草愈伤组织培养的延迟期。使用MTT法评估了所获得的戈亚森内酯对七种癌细胞系的抗增殖活性。结果显示，呋喃合亮内酯具有强大的细胞毒性活性，IC50值范围为：对CEM白血病细胞为0.06微克/毫升，对B16黑色素瘤细胞为0.75微克/毫升。
