Shimada Toshifumi, Nishibe Toshiya, Miura Hidehiko, Hazama Kazuaki, Kato Hiroyuki, Kudo Fabio, Murashita Toshifumi, Okuda Yasuhiro
Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Surg Today. 2004;34(12):1025-30. doi: 10.1007/s00595-004-2862-x.
To evaluate the intermediate performance of small-caliber, long-fibril expanded polytetrafluoroethylene (ePTFE) vascular grafts pretreated with covalent bonding of fibronectin in dogs.
Small-caliber (4 mm), long-fibril (60 microm), ePTFE vascular grafts, 10 cm in length, were pretreated by covalent bonding of fibronectin. Bilateral iliac grafting was done in dogs using a fibronectin-bonded graft on one side and a nonbonded control graft on the other side. The grafts were retrieved 12 weeks after implantation, and subjected to histomorphometric analysis.
Although the patency rates of the fibronectin-bonded and control grafts were the same (3/7, 43%), the fibronectin-bonded grafts showed almost complete neointimal healing, whereas the nonbonded control grafts showed only partial neointimal healing, proximally and distally.
Small-caliber, long-fibril ePTFE vascular grafts with covalent bonding of fibronectin achieved almost complete neointimal healing by the time of retrieval at 12 weeks. This indicates that, with further modifications, our new technique for covalent bonding of fibronectin has great potential in the development of small-caliber arterial prosthetic grafts.
评估经纤连蛋白共价结合预处理的小口径、长纤维膨体聚四氟乙烯（ePTFE）血管移植物在犬体内的中期性能。
对长度为10 cm的小口径（4 mm）、长纤维（60微米）ePTFE血管移植物进行纤连蛋白共价结合预处理。在犬双侧髂动脉进行移植，一侧使用纤连蛋白结合移植物，另一侧使用未结合的对照移植物。植入12周后取出移植物，进行组织形态计量学分析。
尽管纤连蛋白结合移植物和对照移植物的通畅率相同（3/7，43%），但纤连蛋白结合移植物显示出几乎完全的内膜愈合，而未结合的对照移植物仅在近端和远端显示部分内膜愈合。
经纤连蛋白共价结合的小口径、长纤维ePTFE血管移植物在12周取出时实现了几乎完全的内膜愈合。这表明，通过进一步改进，我们的纤连蛋白共价结合新技术在小口径动脉人工移植物的开发中具有巨大潜力。
