Kobayashi Akira, Yoshita Tsuyoshi, Sugiyama Kazuhisa
Department of Ophthalmolog, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa-prefecture 920-8641, Japan.
Ophthalmic Surg Lasers Imaging. 2004 Nov-Dec;35(6):482-4.
To report in vivo laser and white-light confocal microscopic findings of human conjunctiva to investigate the potential application of these confocal microscopes as diagnostic devices for normal and pathologic conjunctiva.
Two healthy volunteers, 28- and 35-year-old men, participated in this study. The temporal bulbar conjunctiva, which was approximately 5 mm away from the limbus, was examined by in vivo laser and white-light confocal microscopic analysis.
Using laser confocal microscopy, at least two different cell types could be distinguished in both subjects: conjunctival superficial epithelial cells and conjunctival basal epithelial cells. In contrast, conjunctival epithelial cells could not be visualized using white-light confocal microscopy.
These results indicate that laser confocal microscopy, but not white-light confocal microscopy, can be used to visualize in vivo human conjunctiva. Further investigations in a large number of normal subjects and in patients with conjunctival pathologies are required to fully evaluate the usefulness of this device in daily clinical ophthalmology.
报告人体结膜的体内激光及白光共聚焦显微镜检查结果，以研究这些共聚焦显微镜作为正常及病理性结膜诊断设备的潜在应用。
两名健康志愿者，分别为28岁和35岁男性，参与了本研究。对距角膜缘约5毫米的颞侧球结膜进行体内激光及白光共聚焦显微镜分析检查。
使用激光共聚焦显微镜，两名受试者均可区分出至少两种不同细胞类型：结膜浅表上皮细胞和结膜基底上皮细胞。相比之下，使用白光共聚焦显微镜无法观察到结膜上皮细胞。
这些结果表明，激光共聚焦显微镜而非白光共聚焦显微镜可用于体内观察人体结膜。需要对大量正常受试者及结膜病变患者进行进一步研究，以充分评估该设备在日常临床眼科中的实用性。
