Efron Nathan, Al-Dossari Munira, Pritchard Nicola
Institute of Health and Biomedical Innovation and School of Optometry, Queensland University of Technology, Kelvin Grove, Queensland, Australia.
Optom Vis Sci. 2009 Nov;86(11):E1303-8. doi: 10.1097/OPX.0b013e3181bc652e.
The aim of this work is to develop a more complete understanding of the in vivo histology of the human palpebral conjunctiva and tarsal plate.
The upper eyelids of 11 healthy human volunteer subjects were everted, and laser scanning confocal microscopy was used to examine the various tissue layers of the palpebral conjunctiva and tarsal plate.
The superficial and basal epithelial layers are composed of cells with gray cytoplasm and thick, light gray borders. Nuclei can not be seen. The stroma has a varied appearance; fibrous tissue is sometimes observed, interspersed with dark, amorphous lacunae, and crevases. Numerous single white or gray cells populate this tissue, and fine blood vessels are seen traversing the field. Occasional conjunctival microcysts and Langerhans cells are observed. The tarsal plate is dark and amorphous, and meibomian gland acini with convoluted borders are clearly observed. Acini are composed of an outer lining of large cuboidal cells, and differentiated secretory cells can be seen within the acini lumen.
Laser scanning confocal microscopy is capable of studying the human palpebral conjunctiva, tarsal plate, and acini of meibomian glands in vivo. The observations presented here may provide useful supplementary anatomical information relating to the morphology of this tissue.
本研究旨在更全面地了解人睑结膜和睑板的体内组织学情况。
翻转11名健康人类志愿者受试者的上眼睑，使用激光扫描共聚焦显微镜检查睑结膜和睑板的各组织层。
浅表上皮层和基底上皮层由细胞质呈灰色、边界厚且为浅灰色的细胞组成，未见细胞核。基质外观多样；有时可见纤维组织，其间散布着深色、无定形的腔隙和褶皱。该组织中有许多单个的白色或灰色细胞，可见细小血管穿过视野。偶尔可见结膜微囊肿和朗格汉斯细胞。睑板呈深色且无定形，可见边界曲折的睑板腺腺泡。腺泡由大的立方体细胞外层构成，腺泡腔内可见分化的分泌细胞。
激光扫描共聚焦显微镜能够在体内研究人睑结膜、睑板和睑板腺腺泡。本文所呈现的观察结果可能为有关该组织形态的解剖学信息提供有用的补充。
