血管内皮生长因子（VEGF）与血管生成素基因在子宫癌肉瘤中的定位

Localization of the VEGF and angiopoietin genes in uterine carcinosarcoma.

作者信息

Emoto Makoto, Charnock-Jones D Stephen, Licence Diana R, Ishiguro Masako, Kawai Michiyasu, Yanaihara Atsushi, Saito Toshiaki, Hachisuga Toru, Iwasaki Hiroshi, Kawarabayashi Tatsuhiko, Smith Stephen K

机构信息

Reproductive Molecular Research Group, Department of Obstetrics and Gynecology, University of Cambridge, The Rosie Maternity Hospital, Robinson Way, Cambridge CB2 2SW, UK.

出版信息

Gynecol Oncol. 2004 Dec;95(3):474-82. doi: 10.1016/j.ygyno.2004.08.042.

DOI:10.1016/j.ygyno.2004.08.042

PMID:15581949

Abstract

OBJECTIVE

Carcinosarcoma of the uterus is a highly aggressive tumor. However, the angiogenesis in this tumor remains unclear. This is the first study to examine the characteristics of angiogenesis in this tumor at the molecular level while also comparing the findings with those of high-grade endometrial carcinoma.

METHODS

The expression of vascular endothelial growth factors (VEGF) and angiopoietins (Ang) genes were examined in 35 primary uterine carcinosarcomas as well as in 12 high-grade endometrial carcinomas by in situ hybridization.

RESULTS

A strong expression of VEGF-A mRNA was significantly seen in carcinosarcomas compared to high-grade endometrial carcinomas. Interestingly, in uterine carcinosarcoma, VEGF-A mRNA was more strongly expressed in the carcinoma cells than in the sarcoma cells. In addition, a decrease in the VEGF-A mRNA expression was found in the transitional areas between carcinomatous and sarcomatous elements in most carcinosarcomas evaluated. Moreover, the Ang-2 mRNA expression was significantly seen in the vasculature adjacent to the periphery of the carcinoma cells in most carcinosarcomas, in comparison to that of endometrial carcinomas.

CONCLUSIONS

A high angiogenic activity in uterine carcinosarcoma was shown, in comparison to that of endometrial carcinoma. Tumor angiogenesis in uterine carcinosarcoma might be chiefly influenced by VEGF-A in the carcinoma cells, in cooperation with Ang-2 in the surrounding microvessels, however, this is not fully usually the case in sarcoma cells.

摘要

目的

子宫癌肉瘤是一种侵袭性很强的肿瘤。然而，该肿瘤中的血管生成情况仍不清楚。这是第一项在分子水平上研究该肿瘤血管生成特征，并将结果与高级别子宫内膜癌进行比较的研究。

方法

通过原位杂交检测35例原发性子宫癌肉瘤以及12例高级别子宫内膜癌中血管内皮生长因子（VEGF）和血管生成素（Ang）基因的表达。

结果

与高级别子宫内膜癌相比，癌肉瘤中VEGF-A mRNA有明显的高表达。有趣的是，在子宫癌肉瘤中，VEGF-A mRNA在癌细胞中的表达比肉瘤细胞中更强。此外，在大多数评估的癌肉瘤中，癌性和肉瘤性成分之间的过渡区域VEGF-A mRNA表达降低。而且，与子宫内膜癌相比，大多数癌肉瘤中癌细胞周边相邻血管中Ang-2 mRNA有明显表达。