Kato Y, Asano K, Mizutani I, Konno T, Sasaki Y, Kutara K, Teshima K, Edamura K, Kano R, Suzuki K, Shibuya H, Sato T, Hasegawa A, Tanaka S
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.
Res Vet Sci. 2006 Oct;81(2):280-6. doi: 10.1016/j.rvsc.2005.12.004. Epub 2006 Mar 20.
The angiopoietin (Ang) family of proteins are central to the regulation of angiogenesis. The purposes of this study were to determine cDNA sequences of canine Ang-1 and Ang-2 and investigate their expressions in normal tissues and spontaneous tumours. The cDNA sequences of canine Ang-1 and Ang-2 were 1,494 and 1,488 bp, and the deduced amino acid sequences were 497 and 495 residues, respectively. The cDNA sequences of canine Ang-1 and Ang-2 showed high homology with those of the other mammalian species. Canine Ang-1 and Ang-2 mRNA were detectable in all 22 normal tissues and spontaneous tumours. Higher mRNA expression level of canine Ang-2 was demonstrated in mammary simple carcinomas, haemangiosarcoma and hepatocellular carcinoma in comparison with normal tissues.
血管生成素（Ang）蛋白家族在血管生成调节中起核心作用。本研究的目的是确定犬血管生成素-1（Ang-1）和血管生成素-2（Ang-2）的cDNA序列，并研究它们在正常组织和自发性肿瘤中的表达。犬Ang-1和Ang-2的cDNA序列分别为1494和1488 bp，推导的氨基酸序列分别为497和495个残基。犬Ang-1和Ang-2的cDNA序列与其他哺乳动物物种的序列具有高度同源性。在所有22种正常组织和自发性肿瘤中均可检测到犬Ang-1和Ang-2 mRNA。与正常组织相比，在乳腺单纯癌、血管肉瘤和肝细胞癌中，犬Ang-2的mRNA表达水平较高。
