Thiele D, Willems H, Krauss H
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen.
Berl Munch Tierarztl Wochenschr. 1992 Feb 1;105(2):45-9.
The current diagnostic procedures for Q fever are discussed, especially isolation and propagation of Coxiella burnetii in BGM cell cultures, detection of antigen by an enzyme immunoassay using a biotinylated monoclonal antibody and the polymerase chain reaction (PCR). The latter is of special importance for differentiation of isolates.
本文讨论了当前Q热的诊断程序,特别是伯氏考克斯体在BGM细胞培养中的分离和繁殖、使用生物素化单克隆抗体通过酶免疫测定法检测抗原以及聚合酶链反应(PCR)。后者对于分离株的鉴别尤为重要。