Q热潜在诊断标志物的鉴定、克隆及表达

Identification, cloning, and expression of potential diagnostic markers for Q fever.

作者信息

Chao C C, Chen H W, Li X, Xu W B, Hanson B, Ching W M

机构信息

Rickettsial Disease Department, Naval Medical Research Center, Silver Spring, MD 20910, USA.

出版信息

Ann N Y Acad Sci. 2005 Dec;1063:76-8. doi: 10.1196/annals.1355.010.

DOI:10.1196/annals.1355.010

PMID:16481493

Abstract

The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, and downstream application.

摘要

Q热的临床诊断较为困难。目前全细胞抗原用于多种血清学方法，但由于伯氏考克斯体培养具有危险性，抗原受到限制。在本报告中，我们描述了利用蛋白质组学技术结合患者血清检测伯氏考克斯体免疫显性抗原的方法，并使用一种以易于表达、纯化和下游应用而闻名的新型载体对所选抗原进行克隆和表达。