Young L J, Deane E M
School of Chemical and Biomedical Sciences, Central Queensland University, Rockhampton 4702, Qld, Australia.
Immunol Lett. 2005 Jan 31;96(2):253-9. doi: 10.1016/j.imlet.2004.09.006.
Monocytes, monocyte-derived adherent cells and dendritic cells all play a role in cellular immunity. In this study, we describe the isolation of monocyte-derived adherent cells and dendritic cells from a model marsupial, the tammar wallaby, Macropus eugenii, and report that in vitro, these cells appear morphologically similar to these cells found in other mammals. The successful culture of marsupial monocyte and dendritic cells was undertaken in serum-free medium which contained lymphocyte conditioned medium as an absolute requirement. This supports the view that similar to cultured dendritic cells from other species reported to date, specific growth factors are required to promote the maturation and differentiation of these cells.
单核细胞、单核细胞衍生的贴壁细胞和树突状细胞在细胞免疫中均发挥作用。在本研究中，我们描述了从一种有袋类动物模型——帚尾袋貂（Macropus eugenii）中分离单核细胞衍生的贴壁细胞和树突状细胞的方法，并报告称在体外，这些细胞在形态上与其他哺乳动物中的同类细胞相似。有袋类单核细胞和树突状细胞的成功培养是在无血清培养基中进行的，该培养基绝对需要淋巴细胞条件培养基。这支持了一种观点，即与迄今报道的来自其他物种的培养树突状细胞类似，这些细胞的成熟和分化需要特定的生长因子。
