Transfusion Medicine and Hemostasis Department, University of Hospital Erlangen, Erlangen, Germany.
Transfus Med Rev. 2010 Apr;24(2):130-9. doi: 10.1016/j.tmrv.2009.11.004.
Leukapheresis is the method of choice to collect monocytes for dendritic cell (DC) culture. Improvement of cell separators and cell collection software have enabled the collection of 10(9) monocytes for the generation of monocyte-derived DCs, which is sufficient to prepare a DC vaccine series. However, leukapheresis works with the technique of differential centrifugation which is not applicable to selectively collect mononuclear cells of similar density. After leukapheresis, thus, additional preparation steps are required to isolate and enrich the desired monocyte population. The cell isolation and cultivation techniques depend on the quality of the original leukocyte harvest due to the monocyte yield and the content of residual erythrocytes and platelets. Monocyte elutriation from the leukapheresis product shows a high monocyte recovery of 80%. However, only 30% of the isolated monocytes can be developed into mature DCs. The factors responsible for DC maturation and the development of different DC subsets are the subject of current research.
白细胞分离术是采集树突状细胞（DC）培养用单核细胞的首选方法。细胞分离机和细胞收集软件的改进，使得能够采集 10(9)个单核细胞来生成单核细胞来源的 DC，这足以制备一系列 DC 疫苗。然而，白细胞分离术采用的差速离心技术不适用于选择性地收集密度相似的单核细胞。因此，在白细胞分离术后，需要额外的制备步骤来分离和富集所需的单核细胞群体。细胞分离和培养技术取决于原始白细胞收获的质量，因为单核细胞的产量和残留红细胞和血小板的含量。从白细胞分离物中洗脱单核细胞显示出 80%的高单核细胞回收率。然而，只有 30%的分离单核细胞可以发育成熟为 DC。负责 DC 成熟和不同 DC 亚群发展的因素是当前研究的主题。
