Yang Yi-da, Zheng Lin, Lu Guo-cai, Chen Ya-gang, Salvato Maria
Department of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2004 Nov;33(6):532-4, 545. doi: 10.3785/j.issn.1008-9292.2004.06.014.
To study the effects of HIV Tat protein on CCR5 expression of monocytes and HIV infection in monocytes.
Membrane expression of CCR5 on monocytes was analyzed by flow cytometry. Stimulated with HIV Tat protein, monocytes were infected with monocyte-tropic HIV(Ba-L) and HIV gag p24 level in the supernatant was measured by ELISA methods.
HIV Tat protein increased CCR5 expression in human monocytes,which was inhibited by rabbit anti-Tat polyclonal antibody. Tat protein also increased p24 level after monocyte-tropic HIV-1(Ba-L) infected monocytes.
Tat increases CCR5 expression and HIV-1 infection in monocytes, which indicates that HIV Tat might be a key protein in HIV-1 infection.
研究HIV Tat蛋白对单核细胞CCR5表达及单核细胞中HIV感染的影响。
采用流式细胞术分析单核细胞表面CCR5的表达。用HIV Tat蛋白刺激单核细胞后，使其感染嗜单核细胞型HIV（Ba-L），并用ELISA法检测上清液中HIV gag p24水平。
HIV Tat蛋白可增加人单核细胞中CCR5的表达，兔抗Tat多克隆抗体可抑制这一作用。Tat蛋白还可使嗜单核细胞型HIV-1（Ba-L）感染单核细胞后p24水平升高。
Tat可增加单核细胞中CCR5的表达及HIV-1感染，这表明HIV Tat可能是HIV-1感染中的关键蛋白。
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