Pittis M G, Prada F, Sternik G, Sen L
Laboratorio de Biología Celular y Retrovirus, Hospital de Pediatría Juan P. Garrahan, Buenos Aires, Argentina.
Viral Immunol. 1996;9(3):169-74. doi: 10.1089/vim.1996.9.169.
The effect of recombinant HIV-1 Tat protein on different functions of peripheral blood monocytes, such as candidacidal activity and phagolysosomal fusion, was evaluated. HIV-1 Tat protein caused a significant impairment of phagolysosomal fusion at 100 ng/ml (p = 0.018). The inhibitory effect of Tat on phagolysosomal fusion was blocked by the addition of 1 microgram/ml monoclonal anti-Tat antibody. Candidacidal activity of peripheral monocytes was not altered by HIV-1 Tat protein at the concentration of 1 microgram/ml. These results indicate the HIV-1 Tat protein can affect the monocyte microbicidal mechanisms at the phagolysosomal fusion step with little or not effect upon the lytic activity.
评估了重组HIV-1 Tat蛋白对人外周血单核细胞不同功能的影响，如杀念珠菌活性和吞噬溶酶体融合。HIV-1 Tat蛋白在100 ng/ml时导致吞噬溶酶体融合显著受损（p = 0.018）。添加1μg/ml单克隆抗Tat抗体可阻断Tat对吞噬溶酶体融合的抑制作用。1μg/ml浓度的HIV-1 Tat蛋白未改变外周单核细胞的杀念珠菌活性。这些结果表明，HIV-1 Tat蛋白可在吞噬溶酶体融合步骤影响单核细胞的杀菌机制，而对裂解活性影响很小或无影响。
