采用两种不同的片形吸虫抗原，通过酶联免疫吸附测定（ELISA）技术评估循环抗片形吸虫IgA抗体。
Evaluation of circulating anti-Fasciola IgA antibodies by ELISA technique using two different Fasciola antigens.
作者信息
Mattar Mona A, El-Toukhy Hanan M
机构信息
Department of Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
出版信息
J Egypt Soc Parasitol. 2004 Dec;34(3):739-49.
Two types of Fasciola antigens: surface tegumental (ST) and excretory-secretory (ES) products of F. gigantica worms were used for performance of ELISA technique. Results showed that, the circulating anti-Fasciola IgA antibodies were detected in 98% and 94% and the specificity of the test was 93.4% and 89.7% using ST and ES antigens, respectively. As regards ST antigen, a highly significant elevation in the level of the circulating anti-Fasciola IgA antibodies was observed according to the intensity of infection as well as in those with hepatomegaly. There was a significant direct relation between the level of circulating anti-Fasciola IgA antibodies and eosinophilia, anaemia and liver function tests of fascioliasis patients when ST antigen was used.
使用两种类型的肝片吸虫抗原
巨片形吸虫虫体的表面皮层（ST）抗原和排泄-分泌（ES）产物进行酶联免疫吸附测定（ELISA）技术。结果显示，使用ST抗原和ES抗原时，循环抗肝片吸虫IgA抗体的检出率分别为98%和94%，检测的特异性分别为93.4%和89.7%。对于ST抗原，根据感染强度以及肝肿大患者，观察到循环抗肝片吸虫IgA抗体水平显著升高。当使用ST抗原时，肝片吸虫病患者的循环抗肝片吸虫IgA抗体水平与嗜酸性粒细胞增多、贫血及肝功能检查之间存在显著的正相关关系。
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