连锁玉米22 kDa α-玉米醇溶蛋白基因的序列分析

Sequence analysis of linked maize 22 kDa alpha-zein genes.

作者信息

Thompson G A, Siemieniak D R, Sieu L C, Slightom J L, Larkins B A

机构信息

Department of Plant Sciences, University of Arizona, Tucson 85721.

出版信息

Plant Mol Biol. 1992 Feb;18(4):827-33. doi: 10.1007/BF00020030.

DOI:10.1007/BF00020030

PMID:1558957

Abstract

We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa alpha-zein genes. The 5' gene (gZ22.8) encodes a complete polypeptide and contains putative regulatory sequences in both the 5' and 3' flanking regions that are typical of zein genes. In contrast, the 3' gene (psi gZ22.8) appears to be a pseudogene, because it contains numerous insertions and deletions that would prevent translation of the mRNA. Alignment of the 5' and 3' flanking sequences of both genes indicated that they resulted from a 3.3 kb DNA duplication event.

摘要

我们已经确定了来自玉米自交系W64A的一个7343 bp玉米醇溶蛋白基因组克隆（gZ22.8H3）的核苷酸序列。对该DNA序列进行计算机辅助分析后发现了两个相邻的22 kDaα-玉米醇溶蛋白基因。5'端基因（gZ22.8）编码一个完整的多肽，并且在5'和3'侧翼区域均含有推测的调控序列，这些序列是玉米醇溶蛋白基因所特有的。相比之下，3'端基因（ψgZ22.8）似乎是一个假基因，因为它包含许多插入和缺失，这会阻止mRNA的翻译。两个基因的5'和3'侧翼序列比对表明，它们是由一个3.3 kb的DNA重复事件产生的。