Maier U G, Brown J W, Tologcyzki C, Feix G
Institute for Biology II, Albert Ludwigs Universität, D-7800 Freiburg, FRG.
EMBO J. 1987 Jan;6(1):17-22. doi: 10.1002/j.1460-2075.1987.tb04712.x.
The genomic organization of the zein structural genes and of regulatory loci influencing their expression suggests that control of zein gene expression will involve interactions between cis elements in the flanking DNA sequences and products from trans-acting genes. The interaction between fragments from the 5' flanking region of a zein gene and specific, double-stranded oligonucleotides with crude nuclear extracts from maize endosperm have been studied by nitrocellulose filter binding, gel retention and DNase I footprinting assays. Specific binding of a nuclear factor was observed and the exact position of the protein binding site was determined. The 22-nt binding site included 14 bp of a 15-bp sequence conserved in all zein genes.
玉米醇溶蛋白结构基因的基因组组织以及影响其表达的调控位点表明，玉米醇溶蛋白基因表达的控制将涉及侧翼DNA序列中的顺式元件与反式作用基因产物之间的相互作用。通过硝酸纤维素滤膜结合、凝胶滞留和DNase I足迹分析，研究了玉米醇溶蛋白基因5'侧翼区域的片段与来自玉米胚乳的粗核提取物的特异性双链寡核苷酸之间的相互作用。观察到一种核因子的特异性结合，并确定了蛋白质结合位点的确切位置。22个核苷酸的结合位点包含了所有玉米醇溶蛋白基因中保守的15个碱基对序列中的14个碱基对。
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