Kim Duk-Soo, Yoo Ki-Yeon, Hwang In-Koo, Jung Ju-Young, Won Moo Ho, Choi Soo-Young, Kwon Oh-Shin, Seo Je Hoon, Kang Tae-Cheon
Department of Anatomy, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea.
Neurosci Res. 2005 Jan;51(1):45-53. doi: 10.1016/j.neures.2004.09.010.
The present study was performed to determine whether the voltage gated Cl- channel (CLC) expression is altered in the hippocampus of seizure sensitive (SS) gerbils, and to identify the strong fast paired-pulse inhibition in the dentate gyrus of SS gerbils is associated with altered CLC expression. In the hippocampal proper and the granule cell layer of the dentate gyrus of the SS gerbils, strong CLC-2 immunoreactivity was detected, as compared with seizure resistant (SR) gerbils. In addition, CLC-3 immunoreactivity was observed in the CA1-3 pyramidal cells, and the granule cell and the molecular layer of the dentate gyrus in the SS gerbils, whereas its immunoreactivity was rarely detected in the SR gerbils. However, CLC-3 immunoreactivity in the mossy fiber was reduced, as compared with SR gerbils. Moreover, infusion of the potential CLC inhibitor (4,4'-diisothiocyanostibene-2,2'-disulfanic acid, DIDS) reduced fast paired-pulse inhibition in the dentate gyrus of SS gerbils, although evoked responses in the dentate gyrus between SR and SS gerbils were similarly detected. These findings suggest that enhancement of CLC expression in the dentate gyrus of SS gerbils may be one of the compensatory responses for reduced GABA(A) receptor-mediated fast postsynaptic inhibitory potentials.
本研究旨在确定癫痫敏感（SS）沙鼠海马中电压门控氯离子通道（CLC）的表达是否发生改变，并确定SS沙鼠齿状回中强烈的快速配对脉冲抑制是否与CLC表达改变有关。与癫痫抵抗（SR）沙鼠相比，在SS沙鼠海马体本身和齿状回的颗粒细胞层中检测到强烈的CLC-2免疫反应性。此外，在SS沙鼠的CA1-3锥体细胞、齿状回的颗粒细胞和分子层中观察到CLC-3免疫反应性，而在SR沙鼠中很少检测到其免疫反应性。然而，与SR沙鼠相比，苔藓纤维中的CLC-3免疫反应性降低。此外，注入潜在的CLC抑制剂（4,4'-二异硫氰基芪-2,2'-二磺酸，DIDS）可降低SS沙鼠齿状回中的快速配对脉冲抑制，尽管在SR和SS沙鼠的齿状回中诱发反应的检测结果相似。这些发现表明，SS沙鼠齿状回中CLC表达的增强可能是对GABA(A)受体介导的快速突触后抑制电位降低的一种代偿反应。
