Mylonas Ioannis, Makovitzky Josef, Shabani Naim, Richter Dagmar-Ulrike, Kuhn Christina, Jeschke Udo, Briese Volker, Friese Klaus
First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Maistrasse 11, 80337 Munich, Germany.
Eur J Obstet Gynecol Reprod Biol. 2005 Jan 10;118(1):101-8. doi: 10.1016/j.ejogrb.2004.06.022.
Leukaemia inhibitory factor (LIF) is a pleiotrophic cytokine, which might play an important role in human reproduction and endocrine-responsive tumours. Therefore, the aim of this study was to determine the frequency and tissue distribution of LIF in normal, hyperplastic and malignant endometrium.
Paraffin-fixed endometrial tissue was obtained from normal premenopausal women (n = 15), endometrial hyperplasia (n = 20), endometroid adenocarcinoma (n = 32) and endometrial polyps (n = 9). The LIF expression was demonstrated by immunohistochemical means and evaluated with a semi-quantitative immunoreactive score. The Mann-Whitney U-test was used for statistical evaluation.
The lowest expression of LIF was observed in endometrial adenocarcinomas compared to all groups, while endometrial polyps expressed the highest LIF immunostaining. The expression in normal human glandular cells was significantly higher during the late secretory phase than in the proliferative phase. The highest expression of LIF was observed in endometrial polyps. Simple hyperplasia showed a significantly higher LIF expression than proliferative endometrium and adenocarcinoma. Adenomatous hyperplasia (AH) grade I-III had a significantly higher LIF expression than adenocarcinoma. The lowest expression of LIF was observed in adenocarcinoma, being statistically significant compared to all groups.
LIF was immunohistochemically demonstrated in normal, hyperplastic and malignant endometrial tissue, suggesting a widespread but complex role for LIF in hyperplastic and malignant endometrial growth regulation. AH I-III also expressed LIF with statistically higher immunostaining than adenocarcinoma. Since AH III can be considered as a precursor of endometrial cancer, LIF could be a marker of cell transformation.
白血病抑制因子（LIF）是一种多效性细胞因子，可能在人类生殖和内分泌反应性肿瘤中发挥重要作用。因此，本研究的目的是确定LIF在正常、增生和恶性子宫内膜中的表达频率及组织分布。
从正常绝经前女性（n = 15）、子宫内膜增生（n = 20）、子宫内膜样腺癌（n = 32）和子宫内膜息肉（n = 9）中获取石蜡包埋的子宫内膜组织。采用免疫组织化学方法检测LIF表达，并通过半定量免疫反应评分进行评估。采用Mann-Whitney U检验进行统计学分析。
与所有组相比，子宫内膜腺癌中LIF表达最低，而子宫内膜息肉中LIF免疫染色最高。正常人腺细胞中LIF表达在分泌晚期明显高于增殖期。LIF在子宫内膜息肉中的表达最高。单纯增生的LIF表达明显高于增殖期子宫内膜和腺癌。I-III级腺瘤样增生（AH）的LIF表达明显高于腺癌。腺癌中LIF表达最低，与所有组相比具有统计学意义。
免疫组织化学证实LIF在正常、增生和恶性子宫内膜组织中均有表达，提示LIF在增生和恶性子宫内膜生长调节中具有广泛但复杂的作用。AH I-III的LIF免疫染色也高于腺癌，具有统计学意义。由于AH III可被视为子宫内膜癌的前驱病变，LIF可能是细胞转化的标志物。
