Mylonas Ioannis, Makovitzky Josef, Shabani Naim, Richter Dagmar-Ulrike, Jeschke Udo, Briese Volker, Friese Klaus
First Department of Obstetrics and Gynecology, Ludwig Maximilians University, Munich, Germany.
Anticancer Res. 2005 May-Jun;25(3A):1633-8.
Parathyroid hormone-related peptide (PTH-rp) is a peptide initially purified from tumors with hypercalcemia, which is also produced in human endometrium. The aim of this study was to determine the frequency and tissue distribution of PTH-rp in normal, hyperplastic and malignant endometrium.
Paraffin-fixed endometrial tissue was obtained randomly from women in the proliferative (n = 4), early secretory (n =5) and late secretory (n = 5) phases, as well as glandular-cystic hyperplasia (n = 5), adenomatous hyperplasia (AH) grade I (n = 5), grade II (n = 4), grade III (n = 4) and endometrioid adenocarcinoma grade I (n = 5). The PTH-rp expression was evaluated by immunohistochemical procedure. A semiquantitative analysis and a statistical evaluation was performed.
Immunohistochemical reaction with PTH-rp was primarily observed in glandular and luminal epithelial cells. Stromal and myometrial cells also expressed PTH-rp. The expression of PTH-rp in glands was significantly higher during the late secretory than in the proliferative phase. The highest expression was observed during AH grade III, while the lowest reaction was detected in the proliferative phase and adenocarcinoma.
PTH-rp was expressed in normal, hyperplastic and malignant endometrial tissue. A cyclical expression of PTH-rp in normal glandular epithelium was observed, being more prominent in the late secretory phase. AH grade I to III also expressed PTH-rp with higher immunostaining than adenocarcinoma. Since AH grade III can be considered as a precursor of endometrial cancer, PTH-rp could be a marker of cell transformation. Endometrioid adenocarcinoma expressed the lowest PTH-rp immunostaining, indicating either a lower expression or a reflection of an increased PTH-rp shedding of malignant transformed endometrium. Further studies are required to establish the usefuleness of PTM-rp as a marker in different endometrial pathologies.
甲状旁腺激素相关肽（PTH-rp）是一种最初从伴有高钙血症的肿瘤中纯化出来的肽，它也在人子宫内膜中产生。本研究的目的是确定PTH-rp在正常、增生性和恶性子宫内膜中的频率及组织分布。
石蜡包埋的子宫内膜组织随机取自处于增殖期（n = 4）、早分泌期（n = 5）和晚分泌期（n = 5）的女性，以及腺囊性增生（n = 5）、I级腺瘤样增生（AH）（n = 5）、II级（n = 4）、III级（n = 4）和I级子宫内膜样腺癌（n = 5）的患者。通过免疫组织化学方法评估PTH-rp的表达。进行了半定量分析和统计学评估。
与PTH-rp的免疫组织化学反应主要见于腺上皮和腔上皮细胞。基质细胞和肌层细胞也表达PTH-rp。腺体中PTH-rp的表达在晚分泌期显著高于增殖期。在III级AH中观察到最高表达，而在增殖期和腺癌中检测到最低反应。
PTH-rp在正常、增生性和恶性子宫内膜组织中表达。在正常腺上皮中观察到PTH-rp的周期性表达，在晚分泌期更明显。I至III级AH也表达PTH-rp，免疫染色高于腺癌。由于III级AH可被视为子宫内膜癌的前体，PTH-rp可能是细胞转化的标志物。子宫内膜样腺癌表达的PTH-rp免疫染色最低，表明要么表达较低，要么反映了恶性转化子宫内膜中PTH-rp脱落增加。需要进一步研究以确定PTH-rp作为不同子宫内膜病变标志物的实用性。
