Ackermans M T, Beckers J L, Everaerts F M, Seelen I G
Laboratory of Instrumental Analysis, Eindhoven University of Technology, Netherlands.
J Chromatogr. 1992 Jan 31;590(2):341-53. doi: 10.1016/0021-9673(92)85397-c.
Reversed-phase high-performance liquid chromatography (RP-HPLC), isotachophoresis (ITP) and capillary zone electrophoresis (CZE) were applied to the determination of salbutamol, terbutaline sulphate and fenoterol hydrobromide in commercially available pharmaceutical dosage forms. The comparison showed that especially with the use of ITP, high concentrations of other charged sample components can disturb the separation process. If special attention is paid to ensure a complete separation, all methods give comparable results. For the regression lines of the calibration graphs, regression coefficients of at least ca. 0.999 and nearly zero intercepts are obtained with relative standard deviations of ca. 1-2% for peak area or zone lengths. By applying the different techniques, often different components of the sample matrix can be detected, i.e., a more complete impression of the sample composition can be obtained by using all the three techniques.
采用反相高效液相色谱法（RP-HPLC）、等速电泳法（ITP）和毛细管区带电泳法（CZE）测定市售药物剂型中的沙丁胺醇、硫酸特布他林和氢溴酸非诺特罗。比较结果表明，尤其是使用ITP时，高浓度的其他带电样品成分会干扰分离过程。如果特别注意确保完全分离，所有方法都能给出可比的结果。对于校准曲线的回归线，获得的回归系数至少约为0.999，截距几乎为零，峰面积或区带长度的相对标准偏差约为1-2%。通过应用不同的技术，通常可以检测到样品基质的不同成分，即使用所有这三种技术可以获得更完整的样品组成印象。
