MacGregor R R, Fowler J S, Wolf A P
Department of Chemistry, Brookhaven National Laboratory, Upton, NY 11973.
J Chromatogr. 1992 Jan 31;590(2):354-8. doi: 10.1016/0021-9673(92)85398-d.
A high-performance liquid chromatographic method has been developed for the quantitation of the enantiomers of cocaine. Mixtures of the naturally occurring (-)-cocaine and synthetically produced (+)-cocaine were hydrolyzed in water to (+) and (-)-benzoyl ecgonine. Esterification with an optically pure 2-octanol resulted in diastereoisomers that could be separated on bare silica gel using an acetonitrile-aqueous ammonium phosphate mobile phase.
已开发出一种高效液相色谱法用于定量可卡因对映体。天然存在的（-）-可卡因和合成生产的（+）-可卡因的混合物在水中水解为（+）和（-）-苯甲酰芽子碱。用光学纯的2-辛醇进行酯化反应，生成非对映异构体，可使用乙腈-磷酸氢铵流动相在硅胶上进行分离。
