Arlian L G, Vyszenski-Moher D L, Lawrence A T, Schrotel K R, Ritz H L
Department of Biological Sciences, Wright State University, Dayton, Ohio 45435.
J Allergy Clin Immunol. 1992 Apr;89(4):866-76. doi: 10.1016/0091-6749(92)90443-6.
The outer portions (husk) of psyllium seeds are a concentrated source of natural fiber used in some bulk-fiber laxatives and cereals. They are known to elicit respiratory allergic reactions after inhalation or ingestion among sensitized individuals. Antigenic and allergenic characterization of three psyllium-seed fractions (husk, endosperm, and embryo) was conducted with crossed immunoelectrophoresis (CIE), crossed radioimmunoelectrophoresis, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the source of psyllium allergenicity. Homologous CIE demonstrated psyllium endosperm and embryo extracts contained seven and four antigens, respectively. Husk extracts were too gelatinous to react by CIE. However, heterologous CIE profiles of endosperm or embryo extracts, reacted with antihusk antibodies, resulted in antigen-antibody precipitin peaks that matched the heavy staining precipitin lines of homologous reactions for endosperm and embryo, respectively. These results indicated that commercial-grade husk, endosperm, and embryo contained similar antigens. Extracts of all three seed components contained antigens that bound IgE antibodies in the sera of 11 psyllium RAST-positive individuals, as determined by crossed radioimmunoelectrophoresis. The few prominent husk protein/peptide bands resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were common in either embryo or endosperm. Immunoblots revealed common IgE reactive bands in all three seed fractions. Microscopic examination of the powdered commercial-grade psyllium (95% pure) revealed it contained endosperm and embryo particles. These immunologic, biochemical, and microscopic findings suggest that other contaminating seed components are primarily responsible for the allergenicity of commercial-grade psyllium-husk powder rather than the husk itself.
亚麻籽外壳是一些容积性纤维泻药和谷物中天然纤维的浓缩来源。已知在致敏个体吸入或摄入后，它们会引发呼吸道过敏反应。采用交叉免疫电泳（CIE）、交叉放射免疫电泳和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳对三种亚麻籽组分（外壳、胚乳和胚）进行抗原性和致敏性表征，以确定亚麻籽致敏性的来源。同源CIE显示亚麻籽胚乳和胚提取物分别含有7种和4种抗原。外壳提取物过于胶状，无法通过CIE反应。然而，胚乳或胚提取物与抗外壳抗体反应的异源CIE图谱，分别产生了与胚乳和胚同源反应的重染色沉淀线相匹配的抗原 - 抗体沉淀峰。这些结果表明商业级外壳、胚乳和胚含有相似的抗原。通过交叉放射免疫电泳测定，所有三种种子成分的提取物均含有能与11名亚麻籽RAST阳性个体血清中的IgE抗体结合的抗原。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分离出的少数突出的外壳蛋白/肽条带在胚或胚乳中很常见。免疫印迹显示所有三种种子组分中均有常见的IgE反应条带。对商业级亚麻籽粉（95%纯）粉末的显微镜检查显示，它含有胚乳和胚颗粒。这些免疫学、生化和显微镜检查结果表明，商业级亚麻籽外壳粉的致敏性主要由其他污染的种子成分而非外壳本身引起。
