Latres E, Fernández-López J A, Remesar X, Alemany M
Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
J Biochem Biophys Methods. 1992 Mar;24(1-2):39-44. doi: 10.1016/0165-022x(92)90044-b.
A specific enzymatic method for the routine measurement of L-leucine in blood samples is presented. The method uses a commercial preparation of tRNAs and amino acetyl-tRNA synthetases for the specific loading of L-leucine into the tRNA(Leu) present, competing with carrier-free L-[U-14C]leucine. The radioimmunoassay-like plot of radioactivity found in the acid-insoluble (tRNA) fraction was used to determine the amount of unlabelled L-leucine of the samples when compared against a standard curve. The interference of L-isoleucine, L-valine and L-alanine was very low.
本文介绍了一种用于常规测量血样中L-亮氨酸的特定酶法。该方法使用市售的tRNA和氨酰-tRNA合成酶制剂，将L-亮氨酸特异性加载到存在的tRNA（Leu）中，与无载体的L-[U-14C]亮氨酸竞争。将酸不溶性（tRNA）部分中发现的放射性类似放射免疫测定的曲线，与标准曲线比较时，用于确定样品中未标记L-亮氨酸的量。L-异亮氨酸、L-缬氨酸和L-丙氨酸的干扰非常低。
