Fernández-López J A, Latres E, Remesar X, Alemany M
Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
J Biochem Biophys Methods. 1993 Jul;26(4):291-7. doi: 10.1016/0165-022x(93)90030-r.
A method is presented for the estimation of L-leucine concentration and radioactivity in biological samples. The sample L-leucine is specifically bound to tRNA and its radioactivity estimated in the presence of either added labelled L-leucine or cold L-leucine (in the same proportion), as well as in the presence of a large excess of cold L-leucine. The latter gave the measurement of non-leucine radioactivity present in the sample. The measurements in the presence and absence of labelled/cold L-leucine allowed the estimation of L-leucine levels and radioactivity by using a simple set of calculations and a standard curve built with known cold L-leucine concentrations in the presence of a fixed known amount of [14C]L-leucine.
本文介绍了一种估算生物样品中L-亮氨酸浓度和放射性的方法。样品中的L-亮氨酸与tRNA特异性结合，在添加标记的L-亮氨酸或冷L-亮氨酸（比例相同）以及大量过量冷L-亮氨酸存在的情况下估算其放射性。后者用于测量样品中存在的非亮氨酸放射性。在有和没有标记/冷L-亮氨酸的情况下进行测量，通过一组简单的计算以及利用在固定已知量的[14C]L-亮氨酸存在下用已知冷L-亮氨酸浓度构建的标准曲线，可估算L-亮氨酸水平和放射性。
