Sakai S, Nakashimada Y, Yoshimoto H, Watanabe S, Okada H, Nishio N
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8530, Japan.
Biotechnol Lett. 2004 Oct;26(20):1607-12. doi: 10.1023/B:BILE.0000045661.03366.f2.
The thermophilic bacterium, Moorella sp. HUC22-1, newly isolated from a mud sample, produced ethanol from H(2) and CO(2) during growth at 55 degrees C. In batch cultures in serum bottles, 1.5 mM ethanol was produced from 270 mM H(2) and 130 mM CO(2) after 156 h, whereas less than 1 mM ethanol was produced from 23 mM fructose after 33 h. Alcohol dehydrogenase and acetaldehyde dehydrogenase activities were higher in cells grown with H(2) and CO(2) than those grown with fructose. The NADH/NAD(+) and NADPH/NADP(+) ratios in cells grown with H(2) and CO(2) were also higher than those in cells grown with fructose. When the culture pH was controlled at 5 with H(2) and CO(2) in a fermenter, ethanol production was 3.7-fold higher than that in a pH-uncontrolled culture after 220 h.
从一份泥浆样本中新分离出的嗜热细菌摩尔氏菌属（Moorella sp.）HUC22 - 1，在55℃生长期间能利用氢气（H₂）和二氧化碳（CO₂）产生乙醇。在血清瓶中的分批培养中，156小时后，由270 mM氢气和130 mM二氧化碳产生了1.5 mM乙醇，而33小时后，由23 mM果糖产生的乙醇不到1 mM。利用氢气和二氧化碳生长的细胞中乙醇脱氢酶和乙醛脱氢酶的活性高于利用果糖生长的细胞。利用氢气和二氧化碳生长的细胞中NADH/NAD⁺和NADPH/NADP⁺的比率也高于利用果糖生长的细胞。当在发酵罐中用氢气和二氧化碳将培养物pH控制在5时，220小时后乙醇产量比未控制pH的培养物高3.7倍。
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