Jia Dechen, Deng Wangshuying, Hu Peng, Jiang Weihong, Gu Yang
CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Bioresour Bioprocess. 2023 Sep 15;10(1):61. doi: 10.1186/s40643-023-00682-z.
In the context of the rapid development of low-carbon economy, there has been increasing interest in utilizing naturally abundant and cost-effective one-carbon (C1) substrates for sustainable production of chemicals and fuels. Moorella thermoacetica, a model acetogenic bacterium, has attracted significant attention due to its ability to utilize carbon dioxide (CO) and carbon monoxide (CO) via the Wood-Ljungdahl (WL) pathway, thereby showing great potential for the utilization of C1 gases. However, natural strains of M. thermoacetica are not yet fully suitable for industrial applications due to their limitations in carbon assimilation and conversion efficiency as well as limited product range. Over the past decade, progresses have been made in the development of genetic tools for M. thermoacetica, accelerating the understanding and modification of this acetogen. Here, we summarize the physiological and metabolic characteristics of M. thermoacetica and review the recent advances in engineering this bacterium. Finally, we propose the future directions for exploring the real potential of M. thermoacetica in industrial applications.
在低碳经济快速发展的背景下，利用天然丰富且成本效益高的一碳（C1）底物来可持续生产化学品和燃料的兴趣日益浓厚。嗜热栖热菌（Moorella thermoacetica）作为一种产乙酸模式细菌，因其能够通过伍德-Ljungdahl（WL）途径利用二氧化碳（CO₂）和一氧化碳（CO），从而在利用C1气体方面展现出巨大潜力，故而备受关注。然而，嗜热栖热菌的天然菌株由于其在碳同化和转化效率方面的局限性以及产品范围有限，尚未完全适用于工业应用。在过去十年中，嗜热栖热菌的遗传工具开发取得了进展，加速了对这种产乙酸菌的理解和改造。在此，我们总结了嗜热栖热菌的生理和代谢特性，并综述了对该细菌进行工程改造的最新进展。最后，我们提出了探索嗜热栖热菌在工业应用中真正潜力的未来方向。
