Faisal Muhammad, Hasnain Shahida
Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan.
Biotechnol Lett. 2004 Nov;26(21):1623-8. doi: 10.1007/s10529-004-3184-1.
Two chromium-resistant bacterial strains, CrT-1 and CrT-13, tolerant up to 40 mg K2CrO4 ml(-1) on nutrient agar, 25 mg ml(-1) in nutrient broth, and up to 10 mg ml(-1) in acetate-minimal media, were identified as Ochrobactrum intermedium and Brevibacterium sp., respectively, on the basis of 16S rRNA gene sequencing. Uptake of chromate was greater in living cells than in heat-killed on dried cells. CrT-1 reduced 82%, 28% and 16% of Cr(VI) at 100, 500, and 1000 microg ml(-1) after 24 h while CrT-13 reduced 41%, 14% and 9%. Other heavy metals at low concentrations did not affect these reductions. At 150 and 300 microg ml(-1) in an industrial effluent sample Cr(VI) was reduced by 87% and 71%, respectively, with CrT-1 and by 68% and 47% with CrT-13.
两种耐铬细菌菌株CrT-1和CrT-13，在营养琼脂上可耐受高达40 mg K2CrO4 ml(-1)，在营养肉汤中可耐受25 mg ml(-1)，在乙酸盐基本培养基中可耐受高达10 mg ml(-1)。根据16S rRNA基因测序，它们分别被鉴定为中间苍白杆菌和短杆菌属。活细胞对铬酸盐的摄取量大于热灭活或干燥细胞。CrT-1在24小时后，对100、500和1000 μg ml(-1)的Cr(VI)的还原率分别为82%、28%和16%，而CrT-13的还原率分别为41%、14%和9%。低浓度的其他重金属不影响这些还原反应。在工业废水样品中，当Cr(VI)浓度为150和300 μg ml(-1)时，CrT-1对其的还原率分别为87%和71%，CrT-13对其的还原率分别为68%和47%。
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