Sultan Sikander, Hasnain Shahida
Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
Bioresour Technol. 2007 Jan;98(2):340-4. doi: 10.1016/j.biortech.2005.12.025. Epub 2006 Feb 20.
A Cr(VI) resistant bacterial strain SDCr-5, identified as Ochrobactrum intermedium on the basis of 16S rRNA gene sequencing, was tolerant to high concentrations of Cr(VI) up to 15 mg ml(-1) in acetate minimal medium. O. intermedium SDCr-5 reduced Cr(VI) under a wide range of concentrations from 100 to 1500 microg ml(-1) and reduction was optimum at 37 degrees C and pH 7. It reduced 200 and 721 microg ml(-1) Cr(VI) within 72 and 96 h, respectively. The rate of Cr(VI) reduction increased with concentration from 100 to 1500 microg ml(-1). The presence of heavy metal cations such as Cu(2+), Co(2+), Mn(2+) and Ni(2+) stimulated Cr(VI) reduction. Strain SDCr-5 might be useful for Cr(VI) detoxification under a wide range of environmental conditions.
一株耐六价铬的细菌菌株SDCr-5,基于16S rRNA基因测序鉴定为中间苍白杆菌,在乙酸盐基本培养基中能耐受高达15 mg ml(-1)的高浓度六价铬。中间苍白杆菌SDCr-5在100至1500 μg ml(-1)的广泛浓度范围内还原六价铬,在37℃和pH 7时还原效果最佳。它分别在72小时和96小时内还原了200和721 μg ml(-1)的六价铬。六价铬的还原速率随着浓度从100至1500 μg ml(-1)而增加。重金属阳离子如Cu(2+)、Co(2+)、Mn(2+)和Ni(2+)的存在刺激了六价铬的还原。菌株SDCr-5在广泛的环境条件下可能对六价铬解毒有用。