Kee Tak W, Cicerone Marcus T
Polymers Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
Opt Lett. 2004 Dec 1;29(23):2701-3. doi: 10.1364/ol.29.002701.
Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its noninvasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by a restricted spectral bandwidth. Recent increases in bandwidth are promising but still are not sufficient for the level of robust component discrimination that would be needed in a chemically complex milieu found, for example, in intracellular and extracellular environments. We demonstrate a truly broadband CARS imaging instrument that we use to acquire hyperspectral images with vibrational spectra over a bandwidth of 2500 cm(-1) with a resolution of 13 cm(-1).
相干反斯托克斯拉曼散射（CARS）显微镜正逐渐成为一种用于对材料和生物系统进行成像的强大方法，部分原因在于其非侵入性和选择性化学敏感性。然而，其在物种选择性成像方面的全部潜力受到有限光谱带宽的限制。最近带宽的增加很有前景，但对于在细胞内和细胞外环境等化学复杂环境中所需的稳健成分区分水平而言仍不充分。我们展示了一种真正的宽带CARS成像仪器，我们用它来获取超光谱图像，其振动光谱带宽为2500 cm⁻¹，分辨率为13 cm⁻¹。
