Yang Lingxiao, Iyer Rishyashring R, Sorrells Janet E, Renteria Carlos A, Boppart Stephen A
Opt Express. 2024 Mar 25;32(7):11474-11490. doi: 10.1364/OE.517417.
Coherent anti-Stokes Raman scattering (CARS) microscopy offers label-free chemical contrasts based on molecular vibrations. Hyperspectral CARS (HS-CARS) microscopy enables comprehensive microscale chemical characterization of biological samples. Various HS-CARS methods have been developed with individual advantages and disadvantages. We present what we believe to be a new temporally optimized and spectrally shaped (TOSS) HS-CARS method to overcome the limitations of existing techniques by providing precise control of the spatial and temporal profiles of the excitation beams for efficient and accurate measurements. This method uniquely uses Fourier transform pulse shaping based on a two-dimensional spatial light modulator to control the phase and amplitude of the excitation beams. TOSS-HS-CARS achieves fast, stable, and flexible acquisition, minimizes photodamage, and is highly adaptable to a multimodal multiphoton imaging system.
相干反斯托克斯拉曼散射（CARS）显微镜基于分子振动提供无标记的化学对比度。高光谱CARS（HS-CARS）显微镜能够对生物样品进行全面的微观化学表征。已经开发出各种HS-CARS方法，各有优缺点。我们提出了一种我们认为是新的时间优化和光谱整形（TOSS）HS-CARS方法，通过精确控制激发光束的空间和时间轮廓来克服现有技术的局限性，以实现高效准确的测量。该方法独特地使用基于二维空间光调制器的傅里叶变换脉冲整形来控制激发光束的相位和幅度。TOSS-HS-CARS实现了快速、稳定和灵活的采集，将光损伤降至最低，并且高度适用于多模态多光子成像系统。
