沉默型血红蛋白变异体与采用高效液相色谱法HA - 8160血红蛋白分析仪的高分辨率程序测定糖化血红蛋白A1c
Silent hemoglobin variants and determination of HbA(1c) with the high-resolution program of the HPLC HA-8160 hemoglobin analyzer.
作者信息
Schnedl Wolfgang J, Lahousen Theresa, Wallner Sandra J, Krause Robert, Lipp Rainer W
机构信息
Department of Internal Medicine, Medical University of Graz, A-8036 Graz, Austria.
出版信息
Clin Biochem. 2005 Jan;38(1):88-91. doi: 10.1016/j.clinbiochem.2004.09.016.
OBJECTIVES
Evaluation of HbA1c determination with an automated ion-exchange high-performance liquid chromatography (HPLC) method in patients with clinically silent hemoglobin (Hb) variants.
DESIGN AND METHODS
HbA1c values were determined with the Arkray HA-8160 ion-exchange HPLC using the high-resolution, 4.2-min beta-thalassemia screening mode in patients with silent hemoglobin (Hb) variants, namely, Hb Graz, Hb Sherwood Forest, Hb O Padova, and HbD.
RESULTS
All of these hemoglobin variants caused additional peaks in the chromatograms, without HbA1c results in patients with Hb Graz and Hb Sherwood Forest, and demonstrated extra peaks with HbA1c results that were clinically too low for patients with Hb O Padova and in the patient with HbD.
CONCLUSIONS
The development of this automated HPLC method modification with high-resolution beta-thalassemia screening mode aids identification of interference due to some clinically silent Hb variants in HbA(1c) determination.
目的
评估采用自动离子交换高效液相色谱（HPLC）法测定临床无症状血红蛋白（Hb）变异患者的糖化血红蛋白（HbA1c）。
设计与方法
使用Arkray HA - 8160离子交换HPLC，采用高分辨率4.2分钟β地中海贫血筛查模式，测定无症状血红蛋白（Hb）变异患者（即Hb Graz、Hb Sherwood Forest、Hb O Padova和HbD）的HbA1c值。
结果
所有这些血红蛋白变异均在色谱图中产生额外峰，Hb Graz和Hb Sherwood Forest患者无HbA1c结果，Hb O Padova患者及HbD患者显示额外峰且HbA1c结果在临床上过低。
结论
这种具有高分辨率β地中海贫血筛查模式的自动HPLC方法改进，有助于识别HbA1c测定中一些临床无症状Hb变异引起的干扰。
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