血红蛋白 E、H 和 G-Taichung 变异体对 Bio-Rad 变异体 II 涡轮分析仪测定 HbA1c 值的影响。
Effects of the Hb E, Hb H and Hb G-Taichung variants on HbA1c values by the Bio-Rad variant II turbo analyzer.
机构信息
Department of Laboratory Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
Clin Biochem. 2011 Nov;44(16):1338-42. doi: 10.1016/j.clinbiochem.2011.08.907. Epub 2011 Aug 17.
OBJECTIVES
The influences of Hb variants on HbA(1c) values can cause mismanagement of diabetes; therefore, the effects of Hb E, H, and G-Taichung variants were evaluated.
DESIGNS AND METHODS
HbA(1c) values of 2105 samples, including 37 samples with Hb E, H and G-Taichung variants identified by Hb electrophoresis and the PCR sequence, were evaluated by the ion-exchange (Bio-Rad Variant II Turbo analyzer) and boronate affinity (Primus CLC 385 analyzer) high performance liquid chromatography (HPLC) methods.
RESULTS
In the patients with the Hb E and H variants, their HbA(1c) values determined by ion-exchange HPLC were significantly higher than those by boronate affinity HPLC. However, there were no significant differences of the HbA(1c) values in the patients with the Hb G-Taichung variant.
CONCLUSIONS
The HbA1c levels might be interfered by the Hb E and H variants, but not the Hb G-Taichung variant, measured by the Bio-Rad Variant II Turbo analyzer.
目的
血红蛋白变异体对 HbA1c 值的影响可能导致糖尿病管理不当；因此，评估了 HbE、H 和 G-台中变异体的影响。
设计和方法
通过血红蛋白电泳和 PCR 序列鉴定的 2105 个样本的 HbA1c 值，包括 37 个 HbE、H 和 G-台中变异体样本，通过离子交换（Bio-Rad Variant II Turbo 分析仪）和硼酸亲和（Primus CLC 385 分析仪）高效液相色谱法（HPLC）进行评估。
结果
在 HbE 和 H 变异体患者中，离子交换 HPLC 测定的 HbA1c 值明显高于硼酸亲和 HPLC 测定的 HbA1c 值。然而，HbG-Taichung 变异体患者的 HbA1c 值无显著差异。
结论
Bio-Rad Variant II Turbo 分析仪测定时，HbE 和 H 变异体可能干扰 HbA1c 水平，但 HbG-Taichung 变异体则不会。
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