Schnedl W J, Reisinger E C, Pieber T R, Lipp R W, Schreiber F, Hopmeier P, Krejs G J
Department of Internal Medicine, Karl Franzens University, Graz, Austria.
Am J Clin Pathol. 1995 Oct;104(4):444-6. doi: 10.1093/ajcp/104.4.444.
Hb Sherwood Forest has been so far identified in only one patient in 1977. This study describes the second detection of this hemoglobin variant by routine high performance liquid chromatography (HPLC) in a diabetic patient and her healthy grand niece. In both, glycosylated hemoglobin (HbA1c) values were excessively elevated (52%), as determined by HPLC with a cation exchange column. The latex agglutination test showed values of HbA1c in the expected normal range. Citrate agar electrophoresis revealed a hemoglobin variant with a mobility similar to HbF. Amino acid analysis and DNA sequence analysis revealed an Arg-->Thr exchange at codon 104 of the beta-chain. This sequence has been described as Hb Sherwood Forest in 1977. The hemoglobin variant is clinically silent and might be confused with excessively high HbA1c in routine measurement of glycosylated hemoglobin.
1977年，仅在一名患者中发现了“舍伍德森林血红蛋白”（Hb Sherwood Forest）。本研究描述了通过常规高效液相色谱法（HPLC）在一名糖尿病患者及其健康的侄孙女中第二次检测到这种血红蛋白变体。通过阳离子交换柱的HPLC测定，两人的糖化血红蛋白（HbA1c）值均过度升高（52%）。乳胶凝集试验显示HbA1c值在预期的正常范围内。柠檬酸盐琼脂电泳显示有一种血红蛋白变体，其迁移率与HbF相似。氨基酸分析和DNA序列分析显示β链第104密码子处发生了精氨酸（Arg）到苏氨酸（Thr）的交换。该序列在1977年被描述为“舍伍德森林血红蛋白”。这种血红蛋白变体在临床上没有症状，在常规糖化血红蛋白测量中可能会与过高的HbA1c混淆。
