Aras Mustafa, Altas Murat, Motor Sedat, Dokuyucu Recep, Yilmaz Atilla, Ozgiray Erkin, Seraslan Yurdal, Yilmaz Nebi
Mustafa Kemal University, Department of Neurosurgery, Hatay, Turkey.
Mustafa Kemal University, Department of Neurosurgery, Hatay, Turkey.
Injury. 2015 Aug;46(8):1471-4. doi: 10.1016/j.injury.2015.05.018. Epub 2015 May 21.
The effects of minocycline on neuronal injury after spinal cord injury (SCI) are limited and controversial. Therefore we aimed to investigate the protective effects of minocycline on tissue and on serum concentrations of malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, tissue total antioxidant and oxidant status (TAS and TOS, respectively), and AST and LDH levels in rats with SCI.
This study was performed on 7-8 weeks 38 male Wistar albino rats. The animals were randomly divided into five groups: group 1, Sham (n=8); group 2, SCI (spinal cord injury)/control (n=8); group 3, SCI+minocycline3 (n=7); group 4, SCI+minocycline30 (n=8) and group 5 SCI+minocycline90 (n=7). Blood and tissue samples were analysed for MDA, SOD, GSH-Px, TAS, TOS, AST and LDH levels.
The MDA levels were significantly higher in SCI group compared to sham group (p<0.001), and MDA levels were also significantly higher in SCI group compared to SCI+M3, SCI+M30, SCI+M90 (p<0.05). SOD levels were significantly higher in SCI+M30 when compared to SCI and SCI+M3 groups (p<0.05). GSH-Px levels decreased significantly in SCI and SCI+M3 groups compared to sham (p<0.05). SCI+M3 group showed significantly decreased levels of TAS and TOS compared to SCI group (p<0.05). TAS and TOS levels significantly increased in SCI+M90 group compared to SCI+M3 and SCI+M30 groups (p<0.05).
The present study demonstrates the dose-dependent antioxidant activity of minocycline against spinal cord injury in rats. Minocycline administration increased antioxidant enzyme levels and improved total antioxidant status.
米诺环素对脊髓损伤（SCI）后神经元损伤的影响有限且存在争议。因此，我们旨在研究米诺环素对SCI大鼠组织以及血清中丙二醛（MDA）水平、超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GSH-Px）活性、组织总抗氧化和氧化状态（分别为TAS和TOS）以及AST和LDH水平的保护作用。
本研究对38只7-8周龄的雄性Wistar白化大鼠进行。动物被随机分为五组：第1组，假手术组（n = 8）；第2组，SCI（脊髓损伤）/对照组（n = 8）；第3组，SCI+米诺环素3mg/kg（n = 7）；第4组，SCI+米诺环素30mg/kg（n = 8）；第5组，SCI+米诺环素90mg/kg（n = 7）。对血液和组织样本进行MDA、SOD、GSH-Px、TAS、TOS、AST和LDH水平分析。
与假手术组相比，SCI组的MDA水平显著更高（p<0.001），与SCI+M3、SCI+M30、SCI+M90组相比，SCI组的MDA水平也显著更高（p<0.05）。与SCI组和SCI+M3组相比，SCI+M30组的SOD水平显著更高（p<0.05）。与假手术组相比，SCI组和SCI+M3组的GSH-Px水平显著降低（p<0.05）。与SCI组相比，SCI+M3组的TAS和TOS水平显著降低（p<0.05）。与SCI+M3组和SCI+M30组相比，SCI+M90组的TAS和TOS水平显著升高（p<0.05）。
本研究证明了米诺环素对大鼠脊髓损伤具有剂量依赖性抗氧化活性。给予米诺环素可提高抗氧化酶水平并改善总抗氧化状态。
