Faculté d'Odontologie, EA3847, CHU, Clermont-Ferrand, France.
J Dent Res. 2010 Jan;89(1):51-5. doi: 10.1177/0022034509353400.
It has not been established whether transmission of mutans streptococci occurs between unrelated children older than 4 years of age. The aim of the study was to investigate the possible transmission of mutans streptococci genotypes from child to child in kindergarten. We studied 96 children (ages 5-6 yrs) in three San Francisco Bay Area public schools. Mutans streptococci colonies from each child were isolated from selective culture on Mitis Salivarius Sucrose Bacitracin agar. We used arbitrary primed polymerase chain reactions to determine the mutans streptococci genotypes. Two children (not siblings) in each of the three schools (6%) shared an identical amplitype of S. mutans, unique to each pair. The 19 S. sobrinus amplitypes were found in 12 children, and all were unique to each child. The presence of matching genotypes of S. mutans demonstrates horizontal transmission of this species between unrelated children aged 5-6 years.
尚未确定 4 岁以上的无亲缘关系儿童之间是否会发生变形链球菌的传播。本研究的目的是调查幼儿园中儿童之间变形链球菌基因型的可能传播。我们研究了三所旧金山湾区公立学校的 96 名儿童（年龄为 5-6 岁）。从 Mitis Salivarius Sucrose Bacitracin 琼脂上的选择性培养物中分离出每个儿童的变形链球菌菌落。我们使用任意引物聚合酶链反应来确定变形链球菌基因型。在每个学校的两个（非兄弟姐妹）儿童（6％）共享一对中各有一个独特的 S. mutans 相同扩增型。在 12 名儿童中发现了 19 个 S. sobrinus 扩增型，并且每个儿童都具有独特性。S. mutans 匹配基因型的存在表明 5-6 岁无亲缘关系儿童之间该物种的水平传播。
