Hurst Robert E, Kamat Chandrashekhar D, Kyker Kimberly D, Green Dixy E, Ihnat Michael A
Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA.
Cancer Lett. 2005 Jan 20;217(2):171-80. doi: 10.1016/j.canlet.2004.07.043.
We have previously shown that growth of bladder carcinoma cell lines onto matrices such as Matrigel and small intestinal submucosal (SIS) gel cause distinct changes in cellular morphology and motility. In these studies, we found that bladder cells grown on Matrigel showed increased resistance to either doxorubicin or mitomycin-C whereas growth of cells in SIS gel caused either significant increases or little difference in drug resistance, depending on both the cells and the drug. Finally, it was found that this altered drug sensitivity is reversible with a finite half-life and is likely due to altered drug accumulation and/or cell cycle kinetics.
我们之前已经表明，膀胱癌细胞系在诸如基质胶和小肠黏膜下层（SIS）凝胶等基质上生长会导致细胞形态和运动性发生明显变化。在这些研究中，我们发现，在基质胶上生长的膀胱细胞对阿霉素或丝裂霉素-C的耐药性增加，而在SIS凝胶中细胞的生长导致耐药性显著增加或变化不大，这取决于细胞类型和药物种类。最后，发现这种改变的药物敏感性具有有限的半衰期，是可逆的，并且可能是由于药物积累和/或细胞周期动力学的改变所致。
