Hauser Paul J, Han Zhiyong, Sindhwani Puneet, Hurst Robert E
Department of Urology, Oklahoma University Health Sciences Center, Oklahoma City, OK 73104, USA.
Anticancer Res. 2007 Mar-Apr;27(2):737-40.
Because the response of cancer cells to chemotherapeutic agents depends upon the supporting extracellular matrix (ECM), the response in vivo may not be reproduced in 2-dimensional cell culture. The dose-response to curcumin and two derivatives by bladder cancer cells grown on both normal (SISgel) and cancer-derived ECM (Matrigel) and on plastic were contrasted. Cells grown on Matrigel were resistant to curcumins, but cells growing on SISgel, which mimic cancer cells suppressed by normal ECM, were nearly as sensitive as cells grown on plastic. SV40-immortalized urothelial cells, which are models for premalignant cells, were the most sensitive, but even aggressive cell lines were nearly as sensitive when grown on SISgel as on plastic. Curcumin response depends highly on the supporting ECM, and cells grown on plastic poorly models cells growing on natural ECM. Curcumin could prove an effective chemopreventive for bladder cancer recurrence when administered intravesically post-therapy.
由于癌细胞对化疗药物的反应取决于支持性细胞外基质（ECM），因此二维细胞培养可能无法重现体内的反应。对比了膀胱癌细胞在正常（SISgel）和癌症衍生的ECM（基质胶）以及塑料上生长时对姜黄素及其两种衍生物的剂量反应。在基质胶上生长的细胞对姜黄素具有抗性，但在SISgel上生长的细胞，类似于被正常ECM抑制的癌细胞，其敏感性几乎与在塑料上生长的细胞相同。SV40永生化尿路上皮细胞是癌前细胞的模型，最为敏感，但即使是侵袭性细胞系在SISgel上生长时也几乎与在塑料上生长时一样敏感。姜黄素反应高度依赖于支持性ECM，在塑料上生长的细胞不能很好地模拟在天然ECM上生长的细胞。在治疗后膀胱内给药时，姜黄素可能被证明是预防膀胱癌复发的有效药物。
