Tenckhoff Solveig, Hollborn Margrit, Kohen Leon, Wolf Sebastian, Wiedemann Peter, Bringmann Andreas
Department of Ophthalmology and Eye Clinic, University of Leipzig Medical Faculty, Liebigstr. 10-14, D-04103 Leipzig, Germany.
Neuroreport. 2005 Jan 19;16(1):53-6. doi: 10.1097/00001756-200501190-00013.
Muller glial cells of the sensory retina mediate K+ and water fluxes that are facilitated by aquaporin-4 (AQP4) water channels and by Kir4.1-K+ channels. However, it is not known which subtypes of aquaporins are expressed in the mammalian retina. Using RT-PCR, we found that both human and rat retinas express mRNA for a diversity of water channel proteins. The human retina expresses mRNAs for AQP0 to AQP12 proteins. Using real-time PCR, we found that the mRNAs for AQP4 and Kir4.1 are downregulated in retinas that were obtained from patients with proliferative retinopathy compared with post-mortem controls. The data suggest that the development of proliferative gliosis is accompanied by disturbed transglial water and ion movements.
感觉视网膜的穆勒神经胶质细胞介导钾离子和水的流动，水通道蛋白4（AQP4）水通道和Kir4.1钾离子通道可促进这种流动。然而，尚不清楚哪种水通道蛋白亚型在哺乳动物视网膜中表达。利用逆转录聚合酶链反应（RT-PCR），我们发现人类和大鼠视网膜均表达多种水通道蛋白的信使核糖核酸（mRNA）。人类视网膜表达AQP0至AQP12蛋白的信使核糖核酸。利用实时聚合酶链反应，我们发现与死后对照相比，增殖性视网膜病变患者视网膜中AQP4和Kir4.1的信使核糖核酸表达下调。数据表明，增殖性胶质增生的发展伴随着跨神经胶质细胞的水和离子运动紊乱。
