Sen Q, Xu Y, Jiang A, Zhang T
Department of Clinical Laboratories, Changhai Hospital, Shanghai 200433.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1997 Jun;11(2):170-3.
This study is to observe the infection of coxsackievirus B3 (Cox B3) to murine monocyte-macrophages (Mo/Mpsi), and its induction of tumor necrosis factor (TNF) and IL-6. Cox B3 was capable of infecting murine macrophages as revealed by immunofluorescence and releasing infectious virus particles. Virus infection with < or = 100 TCID50 did not reduce macrophages viability. Cox B3 stimulated the release of monokines from Mo/Mpsi in vitro and in vivo, markedly increasing the concentration of TNF and IL-6. These were found in the plasma and homogenized heart tissue during the acute phase of murine myocarditis induced by Cox B3, and in the culture supernatant of Mo/Mpsi infected by Cox B3.
本研究旨在观察柯萨奇病毒B3（Cox B3）对小鼠单核细胞-巨噬细胞（Mo/Mpsi）的感染情况，及其对肿瘤坏死因子（TNF）和白细胞介素-6（IL-6）的诱导作用。免疫荧光显示Cox B3能够感染小鼠巨噬细胞，并释放有感染性的病毒颗粒。病毒感染量≤100半数组织培养感染剂量（TCID50）时不会降低巨噬细胞活力。Cox B3在体内外均可刺激Mo/Mpsi释放单核因子，显著增加TNF和IL-6的浓度。在Cox B3诱导的小鼠心肌炎急性期的血浆和心脏匀浆组织中，以及Cox B3感染的Mo/Mpsi培养上清液中均发现了这些物质。
