Dabroś Wojciech, Nikiforuk Agnieszka, Kordowiak Anna M
Department of Clinical and Experimental Pathomorphology, Collegium Medicum, Jagiellonian University, Kraków.
Pol J Pathol. 2004;55(3):15-21.
The authors studied the effect of a short (2 days) oral treatment of rats with bis(kojato)oxovanadium (IV) [VO(ka)2] as 1.8 mmol liquid solution on the biochemical activity and morphology of liver Golgi complexes (Group pVC). Such a short treatment induced greater changes than a longer (1 week) application of the same vanadium compound, what had been observed previously. Especially the Golgi marker enzyme activity (GalT) was the highest among all the investigated groups, and additionally, the greatest dispersion of result was obtained in this group. This group of animals showed twisted Golgi complexes, which--apart from 2-3 narrow cisterns--often contained 1-2 grossly distended cisterns filled with clear, floccular contents. The fairly long cisterns were irregular in shape and were often bent, forming ring-like structures. In the second experiment, we studied the effect of the ligand alone (kojic acid) (Group C+ka2) employed in the same way as in the case of the previously used vanadium complex (time and concentration). The biochemical parameters (body and liver weight, liquid and food intake, blood sugar level and GalT activities) were the same as in the untreated control group (C). Contrary to the biochemical findings, the morphology of Golgi complexes changed in effect of 3.6 mmol kojic acid application (the same application, time and concentration as in the whole complex with vanadium) over a 1-week period, manifesting the stimulation of exocytosis (in the trans region and directed toward plasma membrane), with the cisterns of Golgi dictyosomes being rounded or oval in more than 85% of cases. In this group, electron microscopy revealed the presence of two types of Golgi complexes, namely 2-3 short, slightly arched cisterns grossly distended at both ends and filled with clear, floccular material, as well as vacuoles with the same contents, which were visible in the vicinity of the cisterns. The other type, which was observed less frequently, was represented by Golgi complexes formed by haphazardly twisted cisterns.
作者研究了以1.8 mmol液体溶液形式用双（ kojato ）氧钒（IV）[VO(ka)2]对大鼠进行为期2天的口服治疗对肝脏高尔基体复合物的生化活性和形态的影响（pVC组）。与之前观察到的情况相比，这种短期治疗所引起的变化比使用相同钒化合物进行为期1周的治疗所引起的变化更大。尤其是高尔基体标记酶活性（GalT）在所有研究组中是最高的，此外，该组的结果离散度也是最大的。这组动物的高尔基体复合物出现扭曲，除了2 - 3个狭窄的扁平囊外，还常常包含1 - 2个明显扩张的扁平囊，里面充满了清澈的絮状内容物。相当长的扁平囊形状不规则，常常弯曲，形成环状结构。在第二个实验中，我们研究了单独使用配体（曲酸）的效果（C + ka2组），其使用方式与之前使用的钒配合物相同（时间和浓度）。生化参数（体重、肝脏重量、液体和食物摄入量、血糖水平以及GalT活性）与未治疗的对照组（C组）相同。与生化结果相反，在为期1周的时间内，应用3.6 mmol曲酸（与含钒的整个复合物使用相同的应用方式、时间和浓度）后，高尔基体复合物的形态发生了变化，表现为胞吐作用受到刺激（在反面区域并朝向质膜），在超过85%的情况下，高尔基体扁平囊呈圆形或椭圆形。在该组中，电子显微镜显示存在两种类型的高尔基体复合物，即2 - 3个短的、略微拱形的扁平囊，两端明显扩张，里面充满清澈的絮状物质，以及在扁平囊附近可见的含有相同内容物的液泡。另一种类型较少见，由杂乱扭曲的扁平囊形成的高尔基体复合物代表。
