Dabroś Wojciech, Goc Anna, Turyna Bohdan, Kordowiak Anna M
Department of Pathomorphology, Jagiellonian University Collegium Medicum, Kraków.
Pol J Pathol. 2006;57(2):91-7.
As we have observed previously, rat liver Golgi complexes are very useful cell organelle in investigating the effectiveness of various drugs with cytoprotective or normalizing activities in streptozotocin (STZ)-induced diabetes. The diabetes-associated biochemical and morphological alterations in the organelle were investigated in four groups of rats: control and STZ-diabetic (C and D groups) and compared with the same groups treated with 1.5 mM metavanadate administered as drinking solutions in 0.09 M NaCl over 7 days (C+V and D+V groups). Apart from the untreated group C, a decrease of body weight during the experiment was noted in the remaining three groups, reaching a statistically significant level in the diabetic groups (c. 15%). Fluid and food intake were statistically significantly (p<0.001) limited in both the vanadium treated groups. In the diabetic group treated with metavanadate, the free blood sugar level decreased, but euglycemia was not achieved. In groups C+V, D and D+V, the activity of Golgi marker enzyme, i.e. galactosyltransferase (GalT), was statistically lower as compared with group C (p<0.001). The treatment of diabetic rats with 1.5 mM NaVO3 [V(V)I in 0.09 M NaCl as a drinking solution during 7 days did not normalize the yield of Golgi membrane preparations or the Golgi marker enzyme activity. Electron microscopy revealed marked ultrastructural changes triggered by the employed vanadium compound. A striking change was seen in the presence of giant intracytoplasmic vacuoles. These alterations were seen in both experimental groups, i.e. C+V and D+V. Group C+V showed more advanced ultrastructural changes, what was expressed in a poorer state of mitochondrial membranes, a greater number of vesicular structures and less frequently seen Golgi structures. In spite of the fact that the animals were exposed to two compounds with a strong biological activity, the group of diabetic rats treated with metavanadate (D+V) showed no such advanced changes: more numerous Golgi structures were noted and their form was practically ring-like, i.e. characteristic for this group of organelles.
正如我们之前所观察到的，大鼠肝脏高尔基体在研究各种具有细胞保护或正常化活性的药物对链脲佐菌素（STZ）诱导的糖尿病的疗效方面是非常有用的细胞器。在四组大鼠中研究了该细胞器中与糖尿病相关的生化和形态学改变：对照组和STZ诱导的糖尿病组（C组和D组），并与以0.09M NaCl作为饮用水溶液给予1.5mM偏钒酸盐处理7天的相同组进行比较（C + V组和D + V组）。除了未处理的C组外，其余三组在实验期间体重均下降，在糖尿病组中达到统计学显著水平（约15%）。钒处理组的液体和食物摄入量在统计学上均显著受限（p <0.001）。在用偏钒酸盐处理的糖尿病组中，空腹血糖水平下降，但未实现血糖正常。在C + V组、D组和D + V组中，高尔基体标记酶即半乳糖基转移酶（GalT）的活性与C组相比在统计学上较低（p <0.001）。用1.5mM NaVO3 [V(V)I在0.09M NaCl中作为饮用水溶液处理糖尿病大鼠7天并未使高尔基体膜制剂的产量或高尔基体标记酶活性正常化。电子显微镜显示所使用的钒化合物引发了明显的超微结构变化。在巨大的胞浆内空泡的存在下观察到显著变化。在两个实验组即C + V组和D + V组中均观察到这些改变。C + V组显示出更高级的超微结构变化，表现为线粒体膜状态较差、囊泡结构数量更多且高尔基体结构较少见。尽管动物暴露于两种具有强大生物活性的化合物，但用偏钒酸盐处理的糖尿病大鼠组（D + V）未显示出这种高级变化：观察到更多的高尔基体结构，其形态实际上呈环状，即该组细胞器的特征。
